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KMID : 0378019750180050649
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New Medical Journal
1975 Volume.18 No. 5 p.649 ~ p.653
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Effect of Hypoxia on Thickness of Epiphyseal Cartilage Plate of Femur in Rats
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Abstract
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Young male Sprague Dawley rats of approximately 125-150 grams in body weight at the onset of hypoxia were used.
The animals in the experimental group were housed in a hypoxic environmental chamber which had been fabricated in our laboratory.
Through this chamber, a continuous flow of gas mixture composed of 12.0% oxygen and 88.0% nitrogen was circulated.
Animals of the control group were placed in identical chamber except that an ambient air was made
available for normal respiration.
The experiment was carried out for a period of 7 days and animals of both groups were sacrificed by ;
a blow to the skull. The distal end of femur was removed for subsequent study. At the time of sacrifice, the bone tissues were cleaned the soft tissues and fixed in a Bouin solution for 2 days.
The bone tissues were decalcified in 10% Versene solution for approximately 2 weeks.
The decalcified tissues were then double embedded in parlodion and paraplast in a routine manner. The sections were so oriented that a perfect longitudinal section through antero-posterior axis could
be made.
The sections of 8 micron in thickness on a rotary microtome were carried out by mounting them 1 X 3 slides which had been previously coated with a subbing solution.
The slides were stained in Harris hematoxylin eosin Y.
The thickness of epiphyseal cartilage plate was measured by using caliper divider and photographic enlargement of the slides which were magnified 10 times.
The purpose of the present study was therefore to evaluate the effects of a prolonged hypoxia on thickness of the epiphyseal cartilage plate of femur in rats.
The results were as follows;
1. The hypoxia-treated animals were suppressed the growth of the thickness of epiphyseal cartilage plate in rats.
2. The changes of thickness of the epiphyseal cartilage plate of femur after hypoxic stress may be taken as significant evidence that altered metabolism have occurre l in the chondrocyte of the proliferative zone of the epiphyseal cartilage plate.
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